A novel PPAR alpha/gamma dual agonist inhibits cell growth and induces apoptosis in human glioblastoma T98G cells

• 来源期刊: 中国药理学报（英文版）
• 关键词: TZD18 peroxisome proliferator-activated receptor glioblastoma apoptosis cell division
• 年，卷（期）: 2004,（10）
• 页码范围: 1312-1319
• 页数: 8页
• 语种: 英文

摘要

AIM: To examine the effect of a novel peroxisome proliferator-activated receptor (PPAR) α/γ dual agonist TZD 18 on cell proliferation and apoptosis in human glioblastoma T98G cells and its possible mechanism. METHODS: RTPCR, MTT, TUNEL, Flow cytometry, and Western blot analysis were employed. RESULTS: TZD18 inhibited the growth of T98G cells in a concentration-dependent manner, which was associated with a G1 to S cell cycle arrest.Besides, significant apoptosis was induced after treatment with a non-toxic dose of TZD 18. During the process,the expression of Bcl-2 protein was down-regulated, while that of Bax and p27kip proteins was up-regulated, and the activity of caspase-3 was elevated. However, this effect appeared to be PPARα and PPARγ independent since their antagonists could not reverse this effect. CONCLUSIONS: TZD18, a novel PPARα/γ dual agonist, inhibited cell growth and induce apoptosis in human glioblastoma T98G cells in vitro, indicating a therapeutic potential for TZD 18 in the treatment of glioblastoma.