Effects of esculentoside A on production of interleukin-1,2,and prostaglandin E2

• 来源期刊: 中国药理学报（英文版）
• 关键词: esculentoside A interleukin-1 interleukin-2 prostaglandins E interferon-gamma
• 年，卷（期）: 2004,（6）
• 页码范围: 817-821
• 页数: 5页
• 语种: 英文

摘要

AIM: To investigate the influence of esculentoside A (EsA) on immunological function and its mechanism of antiinflammation. METHODS: Interleukin-1 production was measured by thymocyte co-stimulating assay; the radioactivity of [3H]arachidonic acid (AA) was used to evaluate the release of AA; prostaglandin E2 production was measured with radioimmunoassay (RIA); IL-2 and IFN-γ were detected by ELISA method. RESULTS: EsA (3-12μmol/L)could potently inhibit the production of IL- 1 and PGE2 from both silent and LPS induced macrophages.EsA had no significant effect on the release of AA from murine macrophages. EsA could inhibit the production of IL-2 from murine lymphocytes induced by ConA, but not affect the production from silent lymphocytes. EsA showed no effect on the production of IFN-γ from both silent and ConA induced lymphocytes. CONCLUSION: EsA could affect the immunological function through inhibiting the production of IL-2 from activated splenocytes and the inhibition of production of IL- 1 and PGE2 might be one of the anti-inflammation mechanisms of EsA.