In this paper, a non-autonomous predator-prey model with diffusion and continuous time delay is studied, where the prey can diffuse between two patches of a heterogeneous environment with barriers between patches, but for the predator, the diffusion does not involve a barrier between patches, further it is assumed that all the parameters are time-dependent. It is shown that the system can be made persistent under some appropriate conditions. Moreover,sufficient conditions that guarantee the existence of a unique periodic solution which is globallv asvmptotic stable are derived.