Effect of α-pinene on nuclear translocation of NF-κB in THP-1 cells

• 来源期刊: 中国药理学报（英文版）
• 学科: 医学
• 关键词: alpha-pinene NF-kappa B THP-1 cells I-kappa B fluorescent antibody technique Western blotting
• 年，卷（期）: 2004,（4）
• 页码范围: 480-484
• 页数: 5页
• 分类号: R9
• 语种: 英文

摘要

AIM: To study the effects of α-pinene on nuclear translocation of nuclear factor-κB (NF-κB) and the expression of the inhibitor of NF-κB (Iκ Bα) in human monocyte THP-1 cell line. METHODS: THP-1 cells were incubated with oα-pinene (1, 10, and 100 mg/L, for 30 min) before being stimulated with lipopolysaccharide (LPS, 1 mg/L, 30 min).The location of NF-κB p65 subunit (NF-κB/p65) in THP-1 cells was detected by immunofluorescence and laser scanning confocal microscope (LSCM). The expression of NF-κB/p65 in nuclei and that of IκBα in cytoplasm were measured by Western-blot analysis. RESULTS: The majority of FITC-labelled NF-κB/p65 was located in the nuclei being stimulated with LPS. Whereas, no such fluorescence was seen in the nuclei of the groups pretreated with α-pinene or control cells. α-Pinene pretreatment decreased the NF-κB/p65 nuclear translocation in LPSstimulated THP-1 ceils, and this effect was dose-dependent, but there was no reaction in LPS-unstimulated THP-1 cells. α-Pinene pretreatment increased 1κBα protein level in cytoplasm, compared with that in LPS-stimulated THP- 1 cells. CONCLUSION: In a dose-related fashion, α-pinene inhibits the nuclear translocation of NF-κB induced by LPS in THP-1 cells, and this effect is partly due to the upregulation of IκBα expression.