Investigation on Molecular Non-covalent Interaction in the Sodium Dodecyl Benzene Sulfonatepolychrome Blue B-protein Replacement Reaction

• 来源期刊: 中国化学（英文版）
• 年，卷（期）: 2004,（8）
• 页码范围: 798-803
• 页数: 6页
• 语种: 英文

摘要

The molecular non-covalent interaction often originates from the electrostatic attraction and accords with the Langmuir isothermal adsorption. The sodium dodecyl benzene sulfonate (SDBS)-polychrome blue B (PCB)-protein [bovine serum albumin (BSA), ovalbumin (OVA) and myoglobin (MB)] ternary reaction has been investigated at Ph 3.88. Protein to replace PCB from the PCB-SDBS binding product was used to characterize the assembly of an invisible-spectral compound, SDBS, on proteins by measuring the variation of PCB light-absorption by the microsurface adsorption-spectral correction (MSASC) technique. The effect of ionic strength and temperature on the aggregation was studied. Results showed that the aggregates SDBS92·BSA, SDBS58·OVA and SDBS15·MB at 30 ℃ and SDBS83·BSA, SDBS39·OVA and SDBS10·MB at 50 ℃ are formed.