Effect of Gαq/11 protein and ATP-sensitive potassium channels on prostaglandin E1 preconditioning in rat hearts

• 来源期刊: 中国药理学报（英文版）
• 关键词: G-proteins potassium channels prostaglandin E1 ischemic preconditioning signal transduction ischemia-reperfusion injury
• 年，卷（期）: 2004,（5）
• 页码范围: 587-592
• 页数: 6页
• 语种: 英文

摘要

AIM: To investigate the effect of Gαq/11 signaling pathway and ATP-sensitive potassium channels (KATP channels) on prostaglandin E1 (PGE1) induced early and delay-preconditioning protection in rat hearts. METHODS: Two series of experiments were performed in Wistar rat hearts. In the first series of experiment, all rats were pretreated with PGE1 40 min or 23 h 20 min before the experiment. Ischemia-reperfusion injury was induced by 30 min coronary artery occlusion followed by 90 min reperfusion. Hemodynamics, infarct size, and scores of ventricular arrhythmias were measured. The expression of Gαq/11 protein in the heart was measured by Western blot analysis in the second series. RESULTS: Preconditioning with PGE1 (25 μg/kg ) markedly reduced infarct size, left ventricular enddiastolic pressure, and scores of ventricular arrhythmia. The effect of PGE1 was significantly attenuated by glibenclamide (1 mg/kg, ip), a nonselective KATP channel inhibitor. PGE1 caused a significant increase in the expression of Gαq/11 protein. CONCLUSION: Activations of Gαq/11 signal pathway and KATP channel played significant roles in the cardioprotection of PGE1 preconditioning in rat heart and might be an important mechanism of signal transduction pathway during the PGE1 preconditioning.