Peplomycin induces G1-phase specific apoptosis in liver carcinoma cell line Bel-7402 involving G2-phase arrest

• 来源期刊: 中国药理学报（英文版）
• 关键词: peplomycin Bel-7402 cells apoptosis cyclins cell cycle flow cytometry
• 年，卷（期）: 2004,（12）
• 页码范围: 1698-1704
• 页数: 7页
• 语种: 英文

摘要

AIM: To investigate the mechanism of peplomycin (PEP)-induced apoptosis in liver carcinoma cell line (Bel-7402).METHODS: Growth inhibition by PEP was analyzed using 3- 4,5-Dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) assay. Apoptotic cells were detected using Hoechest 33258 staining, and confirmed by flow cytometric analysis and DNA fragmentation analysis. The expression of cyclin A and B 1 were determined by flow cytometry and Western blot. Annexin V assay was measured by flow cytometric analysis. RESULTS: PEP induced apoptosis and then inhibited cell proliferation in liver carcinoma cell line Bel-7402. Cells treated with PEP 50μmol/L for 15 h were arrested in G2-phase with dramatical expression of cyclin A and a little change in cyclin B1.Almost all the apoptosis occurred in cells undergoing the G1-phase after treatment for 24 h. CONCLUSION:Peplomycin induced G1-phase specific apoptosis in Bel-7402 involving G2-phase arrest.