Effects of paclitaxel on proliferation and apoptosis in human acute myeloid leukemia HL-60 cells

• 来源期刊: 中国药理学报（英文版）
• 学科: 医学
• 关键词: acute promyelocytic leukemia paclitaxel cultured tumor cells genes apoptosis oligonucleotide array sequence analysis cell cycle HL-60 cells
• 年，卷（期）: 2004,（3）
• 页码范围: 378-384
• 页数: 7页
• 分类号: R96
• 语种: 英文

摘要

AIM: To investigate the regulatory effect of paclitaxel on proliferation and apoptosis in human acute leukemia HL60 cells. METHODS: HL-60 cell growth was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tertrazolium bromide (MTT) colorimetric assay. Cell cycle kinetics and apoptosis were analyzed by flow cytometry and microscopic examination. In addition, DNA microarrays containing 14400 EST elements were used to investigate the gene expression pattern of HL-60 cells exposed to paclitaxel 1 μmol/L. RESULTS: Paclitaxel inhibited HL-60 cell growth significantly in a dose-dependent and time-dependent manner (P＜0.01). Marked cell accumulation in G2/M phase and multinucleated cells were also observed after treatment with paclitaxel 0.1 and 1 μmol/L. Among 14400 EST elements, 277 genes were found to be markedly up- or down-expressed in the HL-60 cells treated with paclitaxel 1 μmol/L for 0.5 h, comprising 210 known genes and 67 unknown genes. CONCLUSION: Paclitaxel suppresses the growth of HL-60 cells in vitro by causing cell-cycle arrest and apoptosis. The results of microarray suggest that paclitaxel initiates apoptosis through multiple mechanisms.