Morroniside protects cultured human umbilical vein endothelial cells from damage by high ambient glucose

• 来源期刊: 中国药理学报（英文版）
• 学科: 医学
• 关键词: morroniside umbilical veins endothelial cells diabetic angiopathies
• 年，卷（期）: 2004,（4）
• 页码范围: 412-415
• 页数: 4页
• 分类号: R9
• 语种: 英文

摘要

AIM: To determine whether morroniside, a compound in Comus officinalis Sieb et Zucc can prevent cultured human umbilical vein endothelial cells (HUVEC) from damage by high ambient glucose. METHODS: HUVEC was incubated in glucose, 5 or 30 mmol/L, either alone or in the presence of morroniside (final concentration 100, 10,and 1 μmol/L, respectively) for 48 h. The proliferation of HUVEC was quantified by MTT method; its cycle was analyzed by flow cytometry; morphological change was observed with fluorescence microscopy. RESULTS:Survival of HUVEC cultured in high ambient glucose was significantly decreased when compared to that in normal concentration of glucose (P<0.01). High ambient glucose also lowered the rate of cells entering into S-phase, along with severe morphological damage. With the intervention of morroniside (final concentration 100 and 10 μmol/L),the cell survival was significantly recovered (P<0.01, P<0.05, respectively), accompanied with increased S-phase rate and less extent of morphological damage. CONCLUSION: Morroniside protected HUVEC against high ambient glucose induced injury, which suggested that morroniside could exert a beneficial effect on preventing diabetic angiopathies.