Ionic Liquids as Mobile Phase Additives for Separation of Nucleotides in High-Performance Liquid Chromatography

• 来源期刊: 中国化学（英文版）
• 年，卷（期）: 2004,（6）
• 页码范围: 549-552
• 页数: 4页
• 语种: 英文

摘要

Ionic liquids are a type of salts that are liquid at low temperature (＜ 100 ℃). Because of their some special properties, they have been widely used as new "green solvents" for many chemical reactions and liquid-liquid extraction in the past several years. In this paper, a new method for the separation of nucleotides is developed and the essential feature of the method is that 1-alkyl-3-methylimidazolium salts are used as mobile phase additives, resulting in a baseline separation of nucleotides without need of gradient elution and need of organic solvent addition as currently used in RP-HPLC. This study shows the potential application of ionic liquids as mobile phase additives in reversed-phase liquid chromatography.