Toosendanin increases free-Ca2+ concentration in NG108-15 cells via L-type Ca2+ channels

• 来源期刊: 中国药理学报（英文版）
• 关键词: toosendanin intracellular calcium concentration confocal microscopy calcium indicator neuroblastomaxglioma hybrid cells L-type Ca2+ channels
• 年，卷（期）: 2004,（5）
• 页码范围: 597-601
• 页数: 5页
• 语种: 英文

摘要

AIM: To examine if toosendanin (TSN) affects intracellular free-Ca2+ concentration ([Ca2+]i) in neuroblastomaxglioma hybrid cells (NG108-15 cells). METHODS: The [Ca2+]i was determined by laser-scanning confocal microscopic imaging technique in which Fluo-3 was used as Ca2+ indicator. RESULTS: TSN induced an increase in resting [Ca2+]i and in high K+-evoked Ca2+ transient in differentiated NG108-15 cells. The TSN-induced increase in [Ca2+]i was dose-dependent and disappeared in CdCl2-, nifedipine-containing or Ca2+-free solution, and appeared after washing out the Ca2+ channel blockers or adding Ca2+. CONCLUSION: TSN increased [Ca2+]i in differentiated NG108-15 cells. The [Ca2+]i enhancement was due to the influx of extracellular Ca2+ and related to L-type Ca2+ channels.