Structural Analysis and Identification of Cis-Elements of Rice osRACD Gene

• 来源期刊: 生物化学与生物物理学报（英文版）
• 学科: 生物学
• 关键词: photoperiod-sensitive male genic sterile rice osRACD gene photoperiod fertility transformation cis-element
• 年，卷（期）: 2004,（3）
• 页码范围: 191-198
• 页数: 8页
• 分类号: Q5
• 语种: 英文

摘要

The osRACD gene correlated with fertility transformation in the photoperiod sensitive genic male sterile rice(PGMR),Nongken 58S,encoded a rice(Oryza sativa L.ssp.japonica)small GTPase belonging to the Rac/Rho family.Inverse PCR was performed to amplify a fragment about 1.4 kb in 5′ upstream region of the osRACD promoter.Deletion mutation and gel mobility shift assay characterized two fragments(-799 to -686 nt,and -686 to -431 nt)in the osRACD promoter that could be involved in its transcriptional regulation.When these two deletion fragments were used as probe respectively,a retarded band appeared in the nuclear extracts of fertile 58S rice under short day(58S-SD).Whereas no retarded band was shown in the nuclear extracts of sterile 58S rice under long day(58S-LD).Competition assay indicated that the factors in the retarded bands binding to these two fragments were the same trans-acting factor(termed rice factor,RF).The binding affinity of RF was affected by phosphorylation and was higher in SD-growth rice than that of LD-growth rice.