Effects of a fatty acid synthase inhibitor on adipocyte differentiation of mouse 3T3-L1 cells

• 来源期刊: 中国药理学报（英文版）
• 关键词: adipocytes 3T3-L1 preadipocyte cell differentiation fatty acid synthetase complex C75 cpd peroxisome proliferators-activated receptor γ
• 年，卷（期）: 2004,（8）
• 页码范围: 1052-1057
• 页数: 6页
• 语种: 英文

摘要

AIM: To investigate the influence of C75, a fatty acid synthase inhibitor, on adipocyte differentiation. METHODS:Mouse 3T3-L1 preadipocytes were induced to differentiation by insulin, isobutylmethylxanthine, and dexamethasone.Oil red O staining was performed and activity of glycerol-3-phosphate dehydrogenase (GPDH) was measured. The level of peroxisome proliferators-activated receptor γ (PPARγ) mRNA was assayed by semi-quantitative reverse transcription PCR. RESULTS: C75 blocked the adipogenic conversion in a dose-dependent manner and the inhibitory effects occurred both in the early phases (48 h) and in the latter phases (8 d) of the process. Treatment with C75 for 8 d induced more decrease in lipid content than 48 h (P＜0.01). Treatment with C75 50 mg/L for 48 h or 8 d decreased GPDH activity by 52.8 % and 31.2 % of Vehicle, respectively. Treatment with C75 10-50 mg/L for 48 h or 8 d down-regulated PPARγ mRNA expression compared with control (P＜0.01). CONCLUSION: C75 blocked the adipocyte differentiation, which was related with down-regulation of PPARγ mRNA.