Monosialoganglioside protected ischemic rat hippocampal slices through stabilizing expression of N-methyl-D-aspartate receptor subunit

• 来源期刊: 中国药理学报（英文版）
• 关键词: G(M 1) ganglioside brain hypoxia-ischemia hippocampus spectrophotometry N-methyl-D-aspartate receptors Western blotting
• 年，卷（期）: 2004,（6）
• 页码范围: 727-732
• 页数: 6页
• 语种: 英文

摘要

AIM: To determine direct protective effect of monosialoganglioside (GM1) on hippocampal slices after oxygenglucose deprivation and reperfusion (OGD/RP), and investigate the influence on the expression of N-methyl-Daspartate receptor subunit 1 (NMDAR1) in those hippocampal slices. METHODS: Injury of hippocampal slices and protective effects of GM 1 were detected by 2,3,5-triphenyltetrazolium chloride (TTC) staining, toluidine blue staining, and transmission electron microscopy of rat hippocampal slices. Expression of NMDAR1 was detected by Western blot. RESULTS: (1) GM1 at 1.0 μmol/L was the most effective concentration to preserve the TTC staining of the hippocampal slices after OGD/RP (P＜0.05), and the next was GM1 at 10.0 μmol/L (P＜0.05). (2)Toluidine blue staining and transmission electron microscopy showed GM 1 protected the injuried hippocamal slices after OGD/RP. (3) GM1 downregulated the temporally high expression of NMDAR1 in the hippocampal slices immediately after a 25-min OGD and prevented the over low expression of NMDAR1 after a 30-min reperfusion.CONCLUSION: GM 1 could protect injuried rat hippocampal slices after OGD/RP through stabilizing the expression of NMDAR 1.