D609 induces vascular endothelial cells and marrow stromal cells differentiation into neuron-like cells

• 来源期刊: 中国药理学报（英文版）
• 学科: 医学
• 关键词: tricyclodecane-9-yl-xanthogenate cell differentiation vascular endothelial cells marrow stromal cells neuron-like cells
• 年，卷（期）: 2004,（4）
• 页码范围: 442-446
• 页数: 5页
• 分类号: R9
• 语种: 英文

摘要

AIM: To investigate the effect of tricyclodecane-9-yl-xanthogenate (D609) on cell differentiation in vascular endothelial cells (VECs) and marrow stromal cells (MSCs). METHODS: Morphological changes were observed under phase contrast microscope. Electron microscope and immunostaining were used for VECs identification. The expressions of neuron-specific enolase (NSE) and glial fibrillary acidic protein (GFAP) were examined by immunohistochemistry. RESULTS: After 6 h of induction with D609, some VECs showed morphological changes characteristic of neurones. 9 h later, more VECs became neuron-like cells. About 30.8 % of VECs displayed positive NSE (P<0.01), while the expression of GFAP was negative. When MSCs were exposed to D609, the cells displayed neuronal morphologies, such as pyramidal cell bodies and processes formed extensive networks at 3 h. 6 h later, almost all of the cells exhibited a typical neuronal appearance, and 85.6 % of MSCs displayed intensive positive NSE, but GFAP did not express. CONCLUSION: D609 induces VECs and MSCs differentiation into neuron-like cells.