Compare two methods of measuring DNA damage induced by photogenotoxicity of fluoroquinolones

• 来源期刊: 中国药理学报（英文版）
• 学科: 医学
• 关键词: fluoroquinolones phototoxic dermatitis comet assay
• 年，卷（期）: 2004,（2）
• 页码范围: 171-175
• 页数: 5页
• 分类号: R96
• 语种: 英文

摘要

AIM: To compare two methods of measuring DNA damage induced by photogenotoxicity of fluoroquinolones (FQ). METHODS: Lomefloxacin (LFLX), sparfloxacin (SPFX), ciprofloxacin (CPFX), and levofloxacin (LELX)were tested by comet assay and photodynamic DNA strand breaking activity under the different conditions of UVA irradiation. RESULTS: In comet assay, photogenotoxicity was evident at SPFX 1 mg/L, LFLX 5 mg/L, and CPFX 5 mg/L, and LELX 10 mg/L. In photodynamic DNA srand-breaking activity, SPFX and LFLX induced the conversion of the supercoiled form into the nicked relaxed form at 10-50 μmol/L, while CPFX at 25 μmol/L and LELX at 50 μmol/L. CONCLUSION: There were good correlations between the two methods to detect DNA damage induced by phototoxicity of fluoroquinolones. Photodynamic DNA strand breaking activity was a good method to detect DNA damage induced by photogenotoxicity of fluoroquinolones as well as comet assay.