Effects of Cordyceps militaris extract on angiogenesis and tumor growth

• 来源期刊: 中国药理学报（英文版）
• 关键词: Cordyceps militaris angiogenesis human umbilical vein endothelial cells HT1080 cells B 16-F10 cells matrix metalloproteinases basic fibroblast growth factor
• 年，卷（期）: 2004,（5）
• 页码范围: 657-665
• 页数: 9页
• 语种: 英文

摘要

AIM: To evaluate the effects of Cordyceps militaris extract (CME) on angiogenesis and tumor growth. METHODS:Human umbilical vein endothelial cells (HUVEC), HT1080, and B 16-F10 cells were used. DNA fragment, angiogenic related gene expressions (MMPs, bFGF, VEGF, etc), capillary tube formation, wound healing in vitro, rumor growth in vivo were measured. RESULTS: CME inhibited growth of HUVECs and HT1080 (P＜0.01). CME 100and 200 mg/L reduced MMP-2 gene expression in HT1080 cells by 6.0 % and 22.9 % after 3-h and 14.9 % and 32.8 % after 6-h treatment. CME did not affect MMP-9 gene expression in B16-F10 melanoma cells. CME 100 and 200 mg/L also reduced bFGF gene expression in HUVECs by 22.2 % and 41.3 %. CME inhibited tube formation of endothelial cells in vitro and in vivo. CME repressed the growth of B 16-F10 melanoma cells in mice compared with control group (P＜0.05). CONCLUSION: CME has antiangiogenetic properties.