Melatonin ameliorated okadaic-acid induced Alzheimer-like lesions

• 来源期刊: 中国药理学报（英文版）
• 学科: 医学
• 关键词: Alzheimer disease protein phosphatase okadaic acid neurofilament proteins melatonin
• 年，卷（期）: 2004,（3）
• 页码范围: 276-280
• 页数: 5页
• 分类号: R96
• 语种: 英文

摘要

AIM: To explore the protective effects of melatonin (Mel) on the abnormal phosphorylation of neuronal cytoskeletal proteins. METHODS: We generated a neuroblastoma (SH-SY5Y) cell system in which cytoskeletal proteins are abnormally phosphorylated resulting in microtubule disruption due to the marked inhibition of protein phosphatase activities by okadaic acid (OA). RESULTS: OA-induced declines in cell viability and mitochondrial metabolic activity were remarkably prevented by Mel. In addition, the hyperphosphorylation/accumulation of neurofilament(NF-) H/M subunits and the disruption of microtubules, induced by OA, were significantly inhibited by Mel.CONCLUSION: Our results suggest multiple protective functions of Mel against a series of pathological lesions known to culminate in AD, including abnormal phosphorylation of cytoskeletal proteins, microtubule disassembly and mitochondrion-initiated cell toxicity.