Doxepin protects cultured neurons against oxidative stress-induced injury

• 来源期刊: 中国药理学报（英文版）
• 学科: 医学
• 关键词: doxepin ischemia glutamic acid hemoglobins neurons
• 年，卷（期）: 2004,（3）
• 页码范围: 297-300
• 页数: 4页
• 分类号: R96
• 语种: 英文

摘要

AIM: To investigate protective effects of doxepin (Dox) on cultured neuronal injury induced by oxidative stress.METHODS: Cytotoxicities of glutamate (Glu 0.5 mmol/L, 15 min), sodium dithionite (0.5 mmol/L, 24 h) or hemoglobin (Hb100 mg/L,24 h)and the protective effects of Dox were observed. RESULTS: Exposure of cultured neurons to Glu, sodium dithionite and Hb developed a neurotoxicity expressed in the thiazol blue tetrazolium bromide (MTT) assay, the increase of lactate dehydrogenase (LDH) leakage, malondialdehyde (MDA) content and intracellular [Ca2+]i accumulation, as well as the decrease of superoxide dismutase (SOD) activity. DOX 1-100 nmol/L significantly inhibited all above changes. CONCLUSION: Dox protects cultured neurons against oxidative stress-induced injury by suppressing intracellular [Ca2+]i accumulation, decreasing lipid peroxide generation and stimulating antioxidant enzyme.