Loss of C-terminal α-helix decreased SDF-1α-mediated signaling and chemotaxis without influencing CXCR4 internalization

• 来源期刊: 中国药理学报（英文版）
• 学科: 医学
• 关键词: stromal cell-derived factor-1 alpha CXC chemokine receptor 4 chemotaxis
• 年，卷（期）: 2004,（2）
• 页码范围: 152-160
• 页数: 9页
• 分类号: R96
• 语种: 英文

摘要

AIM: To investigate the possibility that a novel α-helix-defective mutant of stromal cell-derived factor-1α (SDF-1α) (SDF-1/54R) acts as an antagonist of CXC chemokine receptor 4 (CXCR4). METHODS: According to the genetic sequence of natural SDF- 1 α, a recombinant α-helix-defective mutant of SDF- 1 α was designed and some biologic characteristics of this mutant were demonstrated. The migration of Jurkat cells was assessed with chemotactic assay. ERK phosphorylation was analyzed by Western blot with a specific anti-phospho-ERK 1/2 antibody.Intracellular calcium influx was examined by flow cytometer with a calcium indicator dye Fluo-3AM. The CXCR4 on the cell surface was detected by flow cytometer with a PE conjoined anti-human CXCR4 antibody. RESULTS:Compared with native SDF-1α, SDF-1/54R displayed apparent decrease in chemotactic ability, ERK 1/2 activation,and intracellular calcium influx in Jurkat cells. However, the binding to CXCR4 and inducing CXCR4 internalization of SDF-1/54R did not change outstandingly. Moreover, a competitive inhibitory effect of SDF-1/54R on the migration of Jurkat cells induced by native SDF-1 α was confirmed. CONCLUSION: α-helix-defective mutant of SDF-1 α, SDF-1/54R that remained both the N-terminus and the central β-sheet region, decreased SDF-1 α-mediated signaling and chemotaxis but did not influence CXCR4 internalization, which suggested that SDF-1/54R might be developed as an anti-CHIV inhibitor with high biological potency and low side-effect.