A New Method for Determining Dipole Moment Orientation of Single Molecules

• 来源期刊: 中国物理快报（英文版）
• 年，卷（期）: 2004,（5）
• 页码范围: 843-845
• 页数: 3页
• 语种: 英文

摘要

A new imaging method is proposed to determine the three-dimensional dipole moment orientation of single fluorophore. Far-field microscopy can provide orientational information projected in the sample plane, while total internal reflection fluorescence microscopy (TIRFM) can offer the knowledge perpendicular to the surface because longitudinal electric-field components can be generated in total internal reflection geometry. By comparing fluorescence intensities measured with far-field epi-fluorescence microscopy and TIRFM, the exact information of single-fluorescent-molecule orientation is extracted. Detailed analysis of the method is given with a numerical example.