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Aim: To identify proteins that are differentially expressed in cells derived from normal and diseased tunica albuginea (TA) as related to Peyronie's disease (PD). Methods: Cells with characteristics of fibroblasts were isolated from two tissue sources. Those from the plaque of patients with PD were designated as PT cells, and those from the normallyappearing TA of the same patients were designated as NT cells. Messenger RNAs of these cells were analyzed by real-time polymerase chain reaction (RT-PCR) for the expression of monocyte chemoattractant protein 1 (MCP-1).Crude protein lysates were analyzed by surface-enhanced laser desorption/ionization mass spectrometry (SELDIMS) with IMAC30-Cu, CM10, and H50 chips. Each lysate was then separated into six fractions, which were further analyzed by SELDI-MS. Results: RT- PCR analysis showed that PT cells expressed higher levels of MCP-1 than their counterpart NT cells. SELDI-MS analysis showed that the crude protein lysates of all four cell strains produced similar and reproducible protein profiles on IMAC30-Cu and CMl0 chips. Additional SELDI-MS analyses with the fractionated lysates detected three proteins of 11.6 kDa, 14.5 kDa, 22.6 kDa that were upregulated in PT cells and two proteins of 6.3 kDa and 46.9 kDa that were downregulated in PT cells. Conclusion: MCP-1, which is often involved in tissue fibrosis, was expressed at higher levels in PT than that in NT cells. Five potential biomarkers for PD were identified by SELDI-MS analysis.

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中国医学临床医学五官科学内科学医疗保健医药卫生总论基础医学外科学大学学报妇产科学与儿科学特种医学皮肤病学与性病学神经病学与精神病学肿瘤学药学预防医学与卫生学

亚洲男性学杂志(英文版)2005年第4期亚洲男性学杂志(英文版)2005年第3期亚洲男性学杂志(英文版)2005年第2期亚洲男性学杂志(英文版)2005年第1期

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来源期刊	亚洲男性学杂志（英文版）	学科
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年，卷（期）	2005,（3）	所属期刊栏目
研究方向		页码范围	237-243
页数	7页	分类号
字数		语种	英文
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