Triptolide suppresses CD80 and CD86 expressions and IL-12 production in THP-1 cells

• 来源期刊: 中国药理学报（英文版）
• 关键词: triptolide CD80 antigens CD86 antigens interleukin- 12
• 年，卷（期）: 2005,（2）
• 页码范围: 223-227
• 页数: 5页
• 语种: 英文

摘要

Aim: To investigate the effects of triptolide, a diterpenoid triepoxide from Tripterygium wilfordii Hook F (TWHF), on the co-stimulatory molecule expression and interleukin- 12 (IL-12) production from THP-1 cells. Methods: THP-1 cells were differentiated into macrophage-like cells by Me2SO, and then cultured with IFN-γ(500 kU/L) and lipopolysaccharide (LPS) (1 mg/L) with or without triptolide. The surface molecule expressions were analyzed on a FACScan flow cytometer. IL-12p40, IL-12p70 were assayed by ELISA. Results: Tripolide suppressed CD80 and CD86 expressions on IFN-γ (500 kU/L) and LPS (1 mg/L) activated THP-1 cells at nontoxic dosages of 2.5-0.625 μg/L. Furthermore, the production of IL-12p40 and IL-12p70 were also significantly reduced in THP-1 cells exposed to triptolide. Conclusion: Triptolide impairs the antigen-presenting function by inhibiting CD80 and CD86 expressions and decreased IL-12p40 and IL-12p70 (bioactive form) pro ductions from the activated THP-1 cells.