Effects on the quality of frozen-thawed alpaca (Lama pacos)semen using two different cryoprotectants and extenders

• 来源期刊: 亚洲男性学杂志（英文版）
• 年，卷（期）: 2005,（3）
• 页码范围: 303-309
• 页数: 7页
• 语种: 英文

摘要

Aim: To evaluate two extenders and two cryoprotectant agents (CPA) for alpaca semen cryopreservation. Methods:Semen samples were obtained from four adult alpacas (Lama pacos) and frozen using extender Ⅰ (TRIS, citrate, egg yolk and glucose) or extender Ⅱ (skim milk, egg yolk and fructose), each containing either glycerol (G) or ethylene glycol (EG) as CPA. Consequently, four groups were formed: 1) extender Ⅰ-G; 2) extender Ⅰ-EG; 3) extender Ⅱ-G;and 4) extender Ⅱ-EG. Semen was diluted in a two-step process: for cooling to 5 ℃ (extenders without CPA), and for freezing (extenders with CPA). Viability and acrosome integrity were assessed using trypan blue and Giemsa stains. Results: When compared, the motility after thawing was higher (P ＜ 0.05) in groups Ⅱ-EG (20.0 % ± 6.7 %)and Ⅱ-G (15.3 % ± 4.1%) than that in groups Ⅰ-G (4.0 % ± 1.1%) and Ⅰ-EG (1.0 % ± 1.4 %). Viable spermatozoa with intact acrosomes in groups Ⅱ-EG (18.7 % ± 2.9 %) and Ⅱ-G (12.7 % ± 5.9 %) were higher than that in groups Ⅰ-G (5.7 % ± 1.5 %) and Ⅰ-EG (4.0 % ± 1.0 %). Conclusion: The skim milk- and egg yolk-based extenders containing ethylene glycol or glycerol to freeze alpaca semen seems to promote the survival of more sperm cells with intact acrosomes than the other extenders.