The effects of overexpression of Nurrl, a midbrain-specific transcription factor, on SK -N -SH cells vulnerability to neurotoxin 6-OHDA

• 作者: YangLIU Yong-meiZHAO
• 来源期刊: 中国药理通讯
• 学科: 医学
• 关键词: NURRL 中脑 特效药 转录因子 SK-N-SH细胞 神经毒素 6-OHDA PD
• 年，卷（期）: 2005,（2）
• 页码范围: 20
• 页数: 1页
• 分类号: R971

摘要

AIM The aim of this study is to investigate the possible correlation between the expression of Nurrl gene and selective cell death of dopamine (DA) neurons. Nurrl ( nuclear receptor-related factor 1 ) is highly expressed in mesencephalic DA system and specifically required for development and survival of DArgic neurons, the cells primarily lost and aggressive degenerated in Parkinson’s disease (PD). METHODS Firstly, SK-N-SH cells, which do not express endogenous Nurrl, were transfected with the pBK-RSV-Nurrl plasmid by LipofectAMINE and selected by G418. The expression of Nurrl protein in SK-N-SH/Nurrl cells was determined by immunocytochemistry. The effect of Nurrl gene on cell proliferation was also investigated.