Induction of apoptosis in lung cancer cells by isorhamnetin

• 作者: Chun-leiYANG JingXIAO Li-mingZHOU LingZHU Zheng-rongWANG Zun-zhenZHANG
• 来源期刊: 中国药理通讯
• 学科: 医学
• 关键词: 感应现象 细胞凋亡 肺部肿瘤 肿瘤细胞 异鼠李黄素 A549细胞
• 年，卷（期）: 2005,（2）
• 页码范围: 37
• 页数: 1页
• 分类号: R734.2

摘要

AIM The aim of the present study was to explore cytotoxic activity and the mechanism of tumor cell killing by isorhamnetin and to investigate the effect of isorhamnetin on tumor growth, cell prolification and apoptosis in transplantation tumor of lung cancer of Lewis cell line in C57BL/6 mice. METHODS Human A549 cells were treated with 10-320(g/ml isorhamnetin, C57BL/6 mice were subcutaneously inoculated Lewis cells 0.2ml/each (1×107cells/ml) below the right forelimb armpit and were treated with 50 (g/ml isorhamnetin isorhamnetin.The results were observed and analyzed under light-microscope, electronic microscopy, growth inhibition was analyzed by MTT, clonogenic asssays and growth curve;the apoptosis and the expression-associated genes peaks were detected with flow cytometry (FCM), DNA fragmentation, single cell gel electrophoresis (comet) assay,