Effects of {2-[(3-carboxy-1-oxoprogy1)amino]-2-deoxy-D-glucose} on human hepatocellular carcinoma cell line

• 来源期刊: 中国药理学报（英文版）
• 关键词: apoptosis hepatoma HepG2 2-[(3-carboxy-1-oxoprogy 1)amino]-2-deoxy-D-glucose
• 年，卷（期）: 2005,（5）
• 页码范围: 635-640
• 页数: 6页
• 语种: 英文

摘要

Aim: To study the effects of {2-[(3-carboxy-1-oxoprogyl)amino]-2-deoxy-D-glucose (COPADG) on cultured human hepatocellular carcinoma cells (HepG2).Methods: HepG2 cells were cultured in RPMI- 1640 medium. Cell proliferation was determined by MTT assay. Apoptosis was determined by fluorescence microscopy,transmission electron microscopy, agarose gel electrophoresis of DNA fragmenta tion, and flow cytometry. Results: At the concentration ranging between 1-30μmol/L, COPADG potently inhibited the growth and induced apoptosis of HepG2cells. Conclusion: COPADG could effectively induce apoptosis in human hepatocellular carcinoma cells. More investigations are warranted for the potential use of this compound as a new agent for the non-surgical management of human hepatocellular carcinoma.