健脑安对脑缺血再灌注后高糖皮质激素促海马神经细胞凋亡作用的影响

• 作者: 田金洲 魏翠柏
• 来源期刊: 世界急危重病医学杂志
• 学科: 医学
• 关键词: 海马神经细胞 脑缺血再灌注 糖皮质激素 凋亡作用 健脑安 后高
• 年，卷（期）: 2005,（4）
• 页码范围: 797-798
• 页数: 2页
• 分类号: R338

摘要

Stroke is one of three important diseases threaten healthy of human being. Ischemia stroke with high mortality and disability rate is accout for 55%-80% of stroke. Cerebral ischemia reperfusion injury is one mainly pathological mechanism of ischemia stroke. The functional disturbance of the network of neuro-endocrine and immune (NEI) induced by cerebral ischemia reperfusion heighten abnormally glucocorticiod (GCS) in hippocampus and plasm ,and aggravate pathological impairment after cerebral ischemia reperfusion. Recently investigation showed that excessive GCS is one of important pathological factors causing neuron apoptosis in major brain regions such as hippocampus related to cognitive function, which is responsible for vascular dementia (VaD) after stroke. At present, the mechanism induced neuron apoptosis by higher GCS is remained unkown. JianNaoAn with function of BuShenHuoXueHuaTan, is one effective compound of Chinese herbal drug on curing VaD. The proceding experimental result has confirmed the function of JianNaoAn down-modulated genes expression of IL-1 ,CRF related to NEI and relieved impairment of nerve cells induced by cerebral ischemia -reperfusion. We investigate the action about excessive GCS causing neurons apoptosis after cerebral ischemia and the influence of JianNaoAn on this pathological machenism through radioimmunoassay (RIA), immunohistochemistry, in situ hybridization and TUNEL experimental techniques. Furthermore the protective function of JianNaoAn on neuron after cerebral ischemia-reperfusion and potentially pharmacological methanism to prevent and cure post-stroke dementia were revealed. At 2,6,12,24 h of different ischemia-reperfusion points, the concentration of cortisiol of the JianNaoAn treated group in plasm and hippocampus decreased significantly compared with the model group (P<0.05). The number of positive cells is reduced significantly compared with the model group by TUNEL staining method, especially in reperfusion 12 h,the distinction in number of positive cell between the

作者信息

序号	姓名	单位	发文数	被引次数	H指数	G指数
1	魏翠柏		49	331	9.0	17.0