Overexpression of dishevelled-1 attenuates wortmannin-induced hyperphosphorylation of cytoskeletal proteins in N2a cell

• 来源期刊: 中国药理学报（英文版）
• 关键词: Alzheimer disease tau proteins neurofilament wortmannin dishevelled-1
• 年，卷（期）: 2005,（6）
• 页码范围: 679-684
• 页数: 6页
• 语种: 英文

摘要

Aim: To investigate the effect of dishevelled- 1 (DVL- 1) on wortmannin-induced Alzheimer-like hyperphosphorylation of cytoskeletal proteins in mouse neuroblastoma 2a (N2a) cells. Methods: Cultured N2a cells were transitorily transfected with DVL-1 expression plasmid using LipofectamineTM 2000. Western blot and immunofluorescence microscopy were used to measure the phosphorylation of neurofilament and tau. Results: Level of phosphorylated neurofilament at SMI31 epitope and phosphorylated tau determined by PHF-1 was increased at 1 h and 3 h and back to normal at 6 h after wortmannin 1 μmol/L treatment. The highest level of phosphorylated neurofilament and phosphorylated tau was seen at 1 h and 3 h after wortmannin treatment, respectively. When DVL- 1 protein was overexpressed,the hyperphosphorylation of neurofilament at SMI31 and SMI32 epitopes and tau at PHF- 1 (Ser-396/404), M4 (Thr-231/Ser-235), and Tau- 1 (Ser- 198/199/202) epitopes was attenuated. Conclusion: Overexpression of mouse DVL-1 protein inhibits wortmannin-induced hyperphosphorylation of neurofilament and tau in N2a cells.