Regulating expressions of cyclin D1, pRb, and anti-cancer effects of deguelin on human Burkitt's lymphoma Daudi cells in vitro

• 来源期刊: 中国药理学报（英文版）
• 关键词: deguelin Daudi cells cyclin D1 pRb apoptosis
• 年，卷（期）: 2005,（7）
• 页码范围: 873-880
• 页数: 8页
• 语种: 英文

摘要

Aim: To investigate anticancer effects and molecular mechanism of deguelin on human Burkitt' s lymphoma Daudi cells in vitro and compare the cytotoxicities of deguelin on Daudi cells and human peripheral blood monocular cells (PBMC).Methods: The effects of deguelin on the growth of Daudi cells were studied by 3-(4, 5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium (MTT) assay.Apoptosis were dectected through Hoechst 33258 staining and Annexin V/PI double-labeled cytometry.The effect of deguelin on the cell cycle of Daudi cells were studied by a propidium iodide method.The expressions of cyclin D1 and pRb were checked by Western blot.Results: The proliferation of Daudi cells were decreased in deguelin-treated group with a 24-h IC50 value of 51.55 nmol/L.Deguelin induced Daudi cells apoptosis was in a time- and dose-dependent manner.G0/G1 phase increased and S phase decreased in Daudi cells treated with deguelin.With deguelin 0, 5, 10, 20, and 40 nmol/L treatment for 24 h, G0/G1 phase increased from 37.34% to 56.56%, whereas S phase decreased from 37.72% to 21.36%.PBMC was less sensitive to the cytotoxic effect of deguelin than Daudi cells.The expression of cyclin D 1 and pRb protein were decreased sharply in Daudi cells treated with deguelin.Conclusion: Deguelin is able to inhibit the proliferation of Daudi cells by regulating the cell cycle that arrested cells at G0/G1 phase and inducing the cell apoptosis.Moreover, deguelin selectively induced apoptosis of Daudi cells with low toxicity in PBMC.The antitumor effects of deguelin were related to downregulating the expression of cyclin D 1 and pRb protein.