Resolution improvement of electron micrographs by the direct method for biotin-binding protein streptavidin

• 来源期刊: 中国物理（英文版）
• 关键词: electron crystallography direct method protein structure streptavidin
• 年，卷（期）: 2005,（1）
• 页码范围: 1-5
• 页数: 5页
• 语种: 英文

摘要

Direct-method phase extension has been applied to two-dimensional electron diffraction data of the protein streptavidin. Structure-factor amplitudes from electron diffraction were combined with phases from the corresponding electron micrographs. Maximum-entropy discrimination and cluster analysis were used to derive a solution from a large number of random trials. The phase extension from 0.3 to 0.25nm led to substantial improvement of the reconstructed projection image quality.