INTERACTION OF NON-ADHERENT SUSPENDED NEUTROPHILS TO COMPLEMENT OPSONIZED PATHOGENS:A NEW ASSAY USING OPTICAL TRAPS

• 来源期刊: 细胞研究(英文版)
• 学科: 生物学
• 关键词: 巨口 日月
• 年，卷（期）: 2006,（11）
• 页码范围: 887-894
• 页数: 8页
• 分类号: Q2
• 语种: 英文
• DOI: 10.1038/SJ.CR.7310103

摘要

Phagocytosis of opsonized pathogens by circulating non-adherent neutrophils is an essential step in host defense,which when overwhelmed contributes to sepsis.To investigate the role played by ligation of complement receptors CR3and CR4 in non-adherent neutrophils,we designed a novel assay system utilizing dual optical traps,respectively,hold-ing a suspended unactivated cell and presenting a specific ligand-coated bead to the cell surface.We chose anti-CD18 asan example ligand,mimicking the bacterial ops...