Analysis of Phytochelatins and Other Thiol-Containing Compounds by RP-HPLC with Monobromobimane Precolumn Derivatization

• 来源期刊: 中国高等学校学术文摘·化学
• 关键词: phytochelatins thiol-containing compounds RP-HPLC precolumn derivatization monobromobimane Cd hyperaccumulator Sedum alfredii
• 年，卷（期）: 2006,（1）
• 页码范围: 54-58
• 页数: 5页
• 语种: 英文

摘要

In this article,a method for quantitative determination ofphytochelatins(PCn being the classic example)and other thiol-containing compounds in mixed standard solution and plant tissues is presented.Thiols were converted to fluorescent derivatives by precolumn derivatization with monobromobimane.The results showed that PCn and other thiol-containing compounds in standard mixed solutions were rapidly separated within 15 min by using a ACN 0.1% trifluoroacetic acid binary gradient elution.Glutathione was representatively selected to test the precision of this method.The calibration curve was linear in the range of 1.25-160 ng μl-1(regression coefficient r2=0.9999).It was confirmed that this method was rapid,simple,highly sensitive,stable,and had the property of simultaneous determination of PCn and other thiol-containing compounds.This method was applied to determine PCn and other thiol-containing compounds in a Cd hyperaccumulator Sedum alfredii in response to Cd.It was found that no PCn was detected in any tissue at any Cd treatment,suggesting that Cd hyperaccumulation and detoxification in this plant is not based on PC synthesis.