Thrombin-like enzymes from venom gland of Deinagkistrodon acutus:cDNA cloning, mechanism of diversity and phylogenetic tree construction

• 来源期刊: 中国药理学报（英文版）
• 关键词: thrombin-like enzymes structural diversity RNA recombination phylogenetic tree
• 年，卷（期）: 2006,（2）
• 页码范围: 184-192
• 页数: 9页
• 语种: 英文

摘要

Aim: To clone cDNAs of thrombin-like enzymes (TLEs) from venom gland of Deinagkistrodon acutus and analyze the mechanisms by which their structural diversity arose. Methods: Reverse transcription-polymerase chain reaction and gene cloning techniques were used, and the cloned sequences were analyzed by using bioinformatics tools. Results: Novel cDNAs of snake venom TLEs were cloned. The possibilities of post-transcriptional recombination and horizontal gene transfer are discussed. A phylogenetic tree was constructed. Conclusion:The cDNAs of snake venom TLEs exhibit great diversification. There are several types of structural variations. These variations may be attributable to certain mechanisms including recombination.