C333H, a novel PPARα/γ dual agonist, has beneficial effects on insulin resistance and lipid metabolism

• 来源期刊: 中国药理学报（英文版）
• 关键词: C333H peroxisome proliferator-activated receptor insulin resistance lipid metabolism diabetes
• 年，卷（期）: 2006,（2）
• 页码范围: 223-228
• 页数: 6页
• 语种: 英文

摘要

Aim: To examine the effects of novel peroxisome proliferator-activated receptor (PPAR) α/γdual agonist C333H on insulin resistance and lipid metabolism.Methods: An established dual-luciferase reporter gene assay system was used in vitro to test the activity of C333H with respect to the transcription of human PPARα and PPARγ. A preadipocyte differentiation assay and reverse transcription-polymerase chain reaction were used to detect the functional activities of C333H. In db/db mice, the effects of C333H were investigated with respect to lowering of blood glucose and lipid levels. Results: C333H was determined to be a novel PPARα/γ dual agonist because it strongly induced luciferase activity on human PPARα and PPARγ, promoting the differentiation of preadipocytes to adipocytes, and functioning in upregulating the expression of some glucose and lipid metabolic target genes of the PPAR. In addition, C333H efficiently reduced blood lipid and glucose concentrations in db/db diabetic mice. Conclusion: C333H has dual action on both PPARα and PPARγ, and might be of interest for the amelioration of lipid metabolic disorders and insulin resistance associated with type 2 diabetes.