Role of norepinephrine in development of short-term myocardial hibernation

• 来源期刊: 中国药理学报（英文版）
• 关键词: norepinephrine dobutamine myocardial ischemia myocardial reperfusion myocardial stunning myocardium left ventricular function lactic acid coronary circulation
• 年，卷（期）: 2006,（2）
• 页码范围: 158-164
• 页数: 7页
• 语种: 英文

摘要

Aim: To investigate the role of norepinephrine in the development of short-term myocardial hibernation. Methods: Hearts were removed from rats and set up as isometrically beating or short-term hibernation models. The hearts were perfused with modified Krebs-Henseleit buffer under a controlled perfusion pressure. The myocardial ultrastructure was examined, and the content of ATP, phosphocreatine,and glycogen in myocardium, the extent of myocyte apoptosis, and the amount of Bcl-2 and Bax products were determined after 120-min ischemia assessed by TUNEL and immunocytochemistry. Results: There was no significant difference between the reserpinized hearts and the NS control group with respect to heart function, myocardial ultrastructure, ATP, phosphocreatine, or glycogen content,myocyte apoptosis, or amount of Bax or Bcl-2 products. However, relative to the normal saline group, in the norepinephrine-treated hearts, heart function, and myocardial ultrastructure deteriorated significantly, apoptosis and amount of Bax product increased significantly, and the ATP, phosphocreatine, and glycogen content decreased significantly, as did the amount of Bcl-2 product. Conclusion:Myocardial norepinephrine does not contribute to the development of short-term hibernation, but that exogenous NE can induce progressive decreases in coronary flow and cardiac performance, which might result from the increases in apoptosis and necrosis. Norepinephrine may be an important factor in the deterioration of myocardial structure and function during hibernation, and that antiadrenergic treatment may be helpful for the development and sustainment of shortterm myocardial hibernation.