The Effects and Mechanism of GSA on Expression of MCP-1 in Cultured Human Umbilical Vein Endothelial Cells

• 来源期刊: 岭南心血管病杂志（英文版）
• 学科: 医学
• 关键词: Human glycated serum albumin Human umbilical vein endothelial cells Moncyte chemoattractant protein-1 Oxidative stress
• 年，卷（期）: 2007,（1）
• 页码范围: 38-42
• 页数: 5页
• 分类号: R5
• 语种: 英文

摘要

Objectives To investigate the effects and mechanism of glycated serum albumin (GSA) on expression of Monocyte chemoattratant protein-1 (MCP-1) in Endothelial Cells. Methods Human Umbilical Vein Endothelial Cells (HUVEC)are cultured with GSA of different concentrations and interfered by glycosylation products inhibitor Aminoguanidine (AG) and anti-oxidant N-acetylcysteine (NAC), The expression of MCP- 1 are evaluated by Immunocytochemistry and Sandwich ELISA. MDA content and SOD activity are determined by the technique of TBA and XOD respectively. Results GSA can stimulate MCP-1 production and secretion.Immunocytochemistry showed that after HUVECs were cultured with 50 mg/L GSA, expression of MCP-1 in group 4hrs, 8hrs and 12hrs was 1.3, 1.9 and 2.8 fold as much as that in control group (P ＜ 0.01), and there was significant difference among the experiment groups (P ＜ 0.01). Sandwich ELISA showed that expression of MCP-1 in three different groups was 1.6, 2.4 and 3.0 fold as much as that in control group (P ＜ 0.01), and there was significant difference among the experiment groups (P ＜ 0.01 ); GSA can cause the decrease of SOD activity (P ＜ 0.05) and increase of MDA content (P ＜ 0.01); AG and NAC can restrain obviously the expression of MCP-1 of HUVECs stimulated by GSA (P ＜ 0.01); NAC can restrain the effect of GSA on SOD activity and MDA content in HUVECs (P ＜ 0.05). Conclusions GSA can stimulate the expression of MCP-1 of endothelial cells by inducing endothelial cells oxidative stress.