Spectral Studies on the Interaction between Mercuric Ion and ApoCopC

• 来源期刊: 中国化学(英文版)
• 年，卷（期）: 2007,（5）
• 页码范围: 630-634
• 页数: 5页
• 语种: 英文

摘要

The interaction between mercuric ion and apoCopC in the absence or presence of cupric ion was investigated through difference UV spectra in Hepes buffer (10 mmol·L-1) at pH 7.4. The results suggest that mercuric ion can bind to C- and N-terminal binding sites of apoCopC, and the conditional binding constants were calculated to be kN=(6.79±1.12)×106 mol-1·L and kC=(3.06±0.05)×105 mol-1·L. Using urea as a chemical agent, the conformational stabilities of apoCopC and HgN2+ -CopC-HgC2+ were monitored by fluorescence spectrum in Hepes buffer (50 mmol·L-1) at pH 7.4. The free energy of stabilization is (14.69±0.85) and (16.66±0.55) kJ·mol-1, respectively. HgN2+ -CopC-HgC2+ is more stable than apoCopC.