Cloning and functional analysis of the genes involved in signal transduction in tomato Cf-4-Avr4 pathosystem

• 来源期刊: 中国高等学校学术文摘·农学
• 关键词: tomato Cladosporium fulvum hypersensitiveresponse yeast two-hybrid system virus-induced genesilencing
• 年，卷（期）: 2007,（1）
• 页码范围: 52-57
• 页数: 6页
• 语种: 英文

摘要

Hypersensitive response(HR)is one of the most efficient and common resistance mechanisms in plants.Cloning signaling genes are very important to elucidate the resistance mechanisms.A gene in tomato homologous to several resistance proteins in plant was involved in HR and named as RGL(Resistance Gene Like).RGL protein was used as a bait to screen interacting protein(s)from tomato cDNA library through the yeast two-hybrid system.Two interacting proteins were found,which were called as RGLIP-I and RGLIP2(RGL Interacting Protein),respectively.RGLIP-1 is a protein of 291 amino acids with significant homology with thylakoid lumen protein.RGLIP-2 is a protein of 248 amino acids with significant homology with transducin protein.Virus-Induced Gene Silencing(VIGS)of the two genes results in a partial and complete suppression of Avr4induced HR,which indicates that both genes are involved in hypersensitive response.