A Sensitive Derivatization Method for the Determination of the Sugar Composition after Pre-column Reductive Amination with 3-Amino-9-ethylcarbazole (AEC) by High-Performance Liquid Chromatography

• 来源期刊: 中国化学(英文版)
• 年，卷（期）: 2007,（10）
• 页码范围: 1522-1528
• 页数: 7页
• 语种: 英文

摘要

3-Amino-9-ethylcarbazole (AEC) was employed for monosaccharide derivatization. The derivatives can be analyzed by high-performance liquid chromatography (HPLC) with an ultraviolet detection (wavelength: 254 nm).Monosaccharides were quantitatively derivatized with 3-amino-9-ethylcarbazole (AEC) at 70 ℃ for 60 min. The method was linear for all samples over the concentration range tested (r＞0.999), the precision was found to be satisfactory (R.S.D.＜3%), and the recovery ratios were ＞98.62%. The stability analysis showed R.S.D. Was between 1.81%-3.16%. Detection limits for the samples (D-glucose, L-xylose, D-mannose, L-arabinose, and L-rhamnose)ranged from 0.06 to 1.97 ng/mL (S/N=3). Under the optimized derivatization and HPLC conditions, five monosaccharides were well separated using a narrow bore C18 column (250 mm× 4.6 mm) with 0.1 mol/L ammonium acetate containing 25% acetonitrile at a flow rate of 0.5 mL/min. As an application, the method has been successfully applied to the determination of monosaccharide compositions of three polysaccharides SPPA-1, SPPB-1 and SPPC- 1 of Spirulina platensis. This method also has potential application to oligosaccharide or glycan analyses.