Resonance scattering spectral detection of ultratrace IgG using immunonanogold-HauCl4-NH2OH catalytic reaction

• 来源期刊: 中国科学B辑（英文版）
• 年，卷（期）: 2008,（5）
• 页码范围: 440-446
• 页数: 7页
• 语种: 英文
• DOI: 10.1007/s11426-008-0050-3

摘要

Nanogold particles of 10 nm were used to label goat anti-human IgG (GIgG) to obtain nanogold-labeled GIgG (AuGIgG). In a citrate-HCl buffer solution of pH 2.27, AuGIgG showed a strong catalytic effect on the reaction between HAuCl4 and NH2OH to form big gold particles that exhibited a resonance scatter-ing (RS) peak at 796 nm. Under the chosen conditions, AuGIgG combined with IgG to form immuno-complex AuGIgG-IgG that can be removed by centrifuging at 16000 r/min. AuGIgG in the centrifuging solution also showed catalytic effect on the reaction. On those grounds, an immunonanogold catalytic RS assay for IgG was designed. With addition of IgG, the amount of AuGIgG in the centrifuging solution decreased; the RS intensity at 796 nm (/796nm) decreased linearly.