Resistin induces insulin resistance, but does not affect glucose output in rat-derived hepatocytes

• 来源期刊: 中国药理学报（英文版）
• 关键词: resistin hepatocytes insulin resistance suppressor of cytokine signaling 3
• 年，卷（期）: 2008,（1）
• 页码范围: 98-104
• 页数: 7页
• 语种: 英文
• DOI: 10.1111/j.1745-7254.2008.00709.x

摘要

Aim: The aim of the present study was to observe the effects of resistin on insulin sensitivity and glucose output in rat-derived hepatocytes. Methods: The rat hepatoma cell line H4IIE was cultured and stimulated with resistin; supernant glucose and glycogen content were detected. The insulin receptor substrate (IRS)-1 and IRS-2, protein kinase B/Akt, glycogen synthase kinase-3β (GSK-3β), the suppressor of cytokine signaling 3 (SOCS-3) protein content, as well as the phosphorylation status were assessed by Western blotting. Specific antisense oligodeoxynucleotides directed against SOCS-3 were used to knockdown SOCS-3. Results: Resistin induced insulin resistance, but did not affect glucose output in rat hepatoma cell line H4IIE. Resistin attenuated multiple effects of insulin, including insulin-stimulated glycogen synthesis and phosphorylation of IRS, pro-tein kinase B/Akt, as well as GSK-3β. Resistin treatment markedly induced the gene and protein expression of SOCS-3, a known inhibitor of insulin signaling. Furthermore, a specific antisense oligodeoxynucleotide directed against SOCS-3 treatment prevented resistin from antagonizing insulin action. Conclusion: The major function of resistin on liver is to induce insulin resistance. SOCS-3 induc-tion may contribute to the resistin-mediated inhibition of insulin signaling in H4IIE hepatocytes.