Separation,purification and structure elucidation of a polysaccharide from root of Cudrania tricuspidata

• 来源期刊: 中国高等学校学术文摘·化学
• 关键词: root of Cudrania tricuspidata (Carr.) Bur. polysaccharide separation and purification structural characterization
• 年，卷（期）: 2008,（2）
• 页码范围: 209-212
• 页数: 4页
• 语种: 英文

摘要

A water-soluble polysaccharide (CPS-0) was obtained from the root of Cudrania tricuspidata (Carr.)Bur.,by hot water extraction (70℃),deproteination using enzymolysis and Sevag method,precipitation with ethanol,and fractionation through DEAE-Sephadex A-50 chromatography.The purity of CPS-0 was determined by HPLC and the structure was elucidated by monosaccharide composition analysis,methylation analysis,GC,GC-MS,NMR spectral (1H-NMR,13C-NMR,HMQC),UV,IR,and elemental analysis.The CPS-0 was found to contain glucose residues only.The average repeating unit is a decasaccharide having a backbone consisting of 1,41inked α-D-glucopyranosyl residues to which the side chain consisting of terminal and 1,4-1inked α-D-glucopyranosyl residues was attached at position 6 of the branching residues.