Isolation and identification of the thermophilic alkaline desulphuricant strain

• 来源期刊: 中国科学B辑(英文版)
• 年，卷（期）: 2008,（2）
• 页码范围: 158-165
• 页数: 8页
• 语种: 英文
• DOI: 10.1007/s11426-008-0008-5

摘要

A desulfurization strain that belongs to the thermophilic alkaline desulphuricant is designated as strain GDJ-3 and isolated from Inner Mongolia, China.The colony of the strain shows tiny, yellow, or white-yellow, and it becomes henna with the protracting of cultivated time.The cells are bacilliform (0.3 -0.6×1.0-1.2 μm), motive, and Gram negative.The strain GDJ-3 is able to utilize respectively the thiosulphate, sulfate, sulfite, or sulfide as sulfur source, utilize the carbon dioxide as the carbon source, and utilize the ammonium or nitrate as the nitrogen source.According to GenBank data, 16s RNA re-sults of GDJ-3 are in good agreement with Alpha proteobacterrium sp.(97%) and Ochrobactrum sp.(98%).For GDJ-3, the optimum growth temperature is at 45℃, the optimum pH is at 8.5--8.8, and the optimum rocking speed of sorting table is at 150 r/min.Under the optimum culture condition, the cells of the strain can live for about 18 h.In the desulfurization solution, which is prepared according to the composition of DDS solution, the objectionable constituents of sodium thiosulphate and sodium sul-fide were added factitiously, and the bacterial cell concentration was set at 107/mL.After the regenera-tion of the above desulfurization solution by the strain cells, the concentration of sodium thiosulphate was decreased by 14.75 g/L (percentage loss of content 13.21%), the concentration of sodium sulfide was decreased by 0.76 g/L (percentage loss of content 87.36%) in the desulfurization solution in 9.5 hours, and sulfur appeared.Maybe, this kind of strain can be used as the regeneration's bacterial source of DDS solution.