Nanosilver resonance scattering spectral method for determination of hydroxyl radical and its application

• 来源期刊: 中国科学B辑（英文版）
• 年，卷（期）: 2008,（3）
• 页码范围: 226-232
• 页数: 7页
• 语种: 英文
• DOI: 10.1007/s11426-008-0005-8

摘要

Silver nanoparUcles were prepared under a microwave high-pressure condition using citric acid so-dium as a reducer while the excess citrate was removed under high speed centrifugation. There is a resonance scattering (RS) peak at 470 nm for silver nanoparticles. In a pH 4.0 HAc-NaAc buffer solution, hydroxyl radicals from the Fenton reaction can oxidize silver nanoparticles to Ag+, resulting in the RS intensity decreasing. The decreased RS intensity at 470 nm (△/470nm) is linear with respect to the con-centration of H2O2 (C) in the range of 0.27-7.56 μmol/L with a detection limit of 0.23 μmol/L. Its regres-sion equation is △/470nm= 24.3 C + 13.8 with a correlation coefficient of 0.9959. This method was applied to screening the antioxidants with satisfactory results.