CONSTRUCTION OF RECOMBINANT ADENO-ASSOCIATED VIRUS VECTOR CO-EXPRESSING HVEGF165 AND HBMP7 GENE

• 来源期刊: 南京医科大学学报(英文版)
• 学科: 医学
• 年，卷（期）: 2008,（4）
• 页码范围: 205-210
• 页数: 6页
• 分类号: R5
• 语种: 英文

摘要

Objective: To construct recombinant adeno-associated virus co-expressing human vascular epithelial growth factor 165(hVEGF165) and bone morphogenetic protein 7(hBMP7),measure the virus titer and verify the recombination. Methods:The AAV helper-free system was used as basis to generate recombinant AAV. The IRES sequence of plasmid pIRES was cut down and subcloned into ITR/MCS containing vector pAAV-MCS to construct recombinant plasmid pAAV-MCSa-IRES-MCSb. The hVEGF165 and hBMP7 gene was amplified by PCR and ...