Establishment of transgenic acceptor and transformation of barnase gene by particle gun in maize inbred line 18-599(white)

• 来源期刊: 中国高等学校学术文摘·农学
• 关键词: maize inbred line transgenic acceptor particle gun molecular detection
• 年，卷（期）: 2008,（1）
• 页码范围: 37-43
• 页数: 7页
• 语种: 英文
• DOI: 10.1007/s11703-008-0019-1

摘要

The efficient acceptors for maize transgenic engineering are currently insufficient in China. Seed production by male sterility is the best method for advancing the authenticity of maize hybrid. Maize inbred line 18-599 (white) is an antivirus high-quality maize inbred line in China, which has been used for lots of maize hybrid cultivars. The establishment of high efficiency transgenic acceptors is necessary for advancing the transgenic efficiency in maize transformation work. In this study, the efficient transgenic acceptors were optimized and established. 18-599 (white) was studied in state, types of culture mediums, times of callus regen-eration and concentration of the screening reagent, Basta. The results showed that N6-4 medium was the best in 8 types of mediums for the immature embryo of 18-599 (white), 1.6 mm length was the feasible length of immature embryos for tissue culture in establishing the transgenic acceptor system, and it was within 5 times for suitable callus subculture. With the optimized transgenic acceptors, barnase gene was translated successfully into 18-599 (white) by a particle gun using bar as a marker gene. Basta was used as the screening reagent, its lethal callus regeneration, respectively. In this work, a trans-genic plant with male sterility was obtained through molecule detection and observation in the field. The result has an important significance for the creation of new male sterility inbred lines in maize in the future.