Tetrandrine suppresses lipopolysaccharide-induced microglial activation by inhibiting NF-κB pathway

• 来源期刊: 中国药理学报（英文版）
• 关键词: tetrandrine microglia nitric oxide superoxide anion cytokine NF-κB
• 年，卷（期）: 2008,（2）
• 页码范围: 245-251
• 页数: 7页
• 语种: 英文
• DOI: 10.1111/j.1745-7254.2008.00734.x

摘要

Aim: Microglial activation has been implicated in many neurological diseases. In this study, we examined the effects of tetrandrine (TET), a major pharmacologi-cally-active compound of Chinese herb Stephania tetrandra S Moore on micro-glial activation. Methods: The microglia pretreated with or without TET were activated by lipoopolysaccharide (LPS) in vitro. Nitric oxide (NO) release, superox-ide anion (O2-) generation, as well as TNF-α and intedeukin-6 (IL-6) production by microglia were measured afterwards. Electrophoretic mobility shift assay was performed to determine whether NF-κB activity in microglia was affected by TET treatment. Results: We found that TET inhibited the LPS-induced activation of microglia by decreasing the production of NO and O2-, consequently affecting the release of TNF-α and IL-6 in LPS-induced microglial activation. Such suppressive effect was accompanied by inhibiting transcription factor NF-κB activation. Conclusion: Our results suggest that TET might modulate LPS-induced microglial activation by inhibiting the NF-κB-mediated release of inflammatory factors.