Characterization of microRNAs in serum:a novel class of biomarkers for diagnosis of cancer and other diseases

• 来源期刊: 细胞研究（英文版）
• 年，卷（期）: 2008,（10）
• 页码范围: 997-1006
• 页数: 10页
• 语种: 英文
• DOI: 10.1038/cr.2008.282

摘要

Dysregulated expression of microRNAs (miRNAs) in various tissues has been associated with a variety of diseases,including cancers.Here we demonstrate that miRNAs are present in the serum and plasma of humans and other animals such as mice,rats,bovine fetuses,calves,and horses.The levels of miRNAs in serum are stable,reproduc ible,and consistent among individuals of the same species.Employing Solexa,we sequenced all serum miRNAs of healthy Chinese subjects and found over 100 and 91 serum miRNAs in male and female subjects,respectively.We also identified specific expression patterns of serum miRNAs for lung cancer,colorectal cancer,and diabetes,provid ing evidence that serum miRNAs contain fingerprints for various diseases.Two non-small cell lung cancer-specific se-rum miRNAs obtained by Solexa were further validated in an independent trial of 75 healthy donors and 152 cancer patients,using quantitative reverse transcription polymerase chain reaction assays.Through these analyses,we conclude that serum miRNAs can serve as potential biomarkers for the detection of various cancers and other diseases.