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摘要:
Aim: The aim of the present study was to explore the mechanism for the Ca2+-dependent inactivation of the canonical transient receptor potential (TRPC) 7 channel expressed in human embryonic kidney 293 cells. Method: The whole-cell patch-clamp technique was used in the study. Results: With Ca2+-free external solution, the perfusion of 100 μmol/L carbachol to, or dialysis of the cell with 100 μnol/L guanosine 5'-3-O-(thio)triphosphate (GTPγS), induced large inward currents, respectively. These currents were rapidly inhibited by the addition of 1 mmol/L Ca2+ into the bath, and recovery from this inhibition was only partial after the Ca2+ removal, unless vigorous intracellular Ca2+ buffering with 10 mmol/L 1,2 bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA) (plus 4 mmol/L Ca2+) was employed. In contrast, the current induced by a membrane-permeable analog of diacylglycerol (DAG), 1-oleoyl-2-acetyl-sn-glycerol (OAG; 100 μnol/L) did not undergo the inhibition persisting after Ca2+ removal. Interestingly, the inclusion of inositol 1,4,5 trisphosphate (IP3; 100 μmol/L) in the patch pipette rendered the OAG-induced current susceptible to the persistent Ca2+-mediated inhibition inde-pendent of the IP3 receptor in the majority of the tested cells, as evidenced by the inability of heparin and thapsigargin in reversing the effect of IP3. Conclusion: The present results suggest that Ca2+ entry via the activated TRPC7 channel plays a critical role in inactivating the channel where the cooperative actions of DAG and IP3 are essentially involved.
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篇名 Synergistic actions of diacylglycerol and inositol 1,4,5 trisphosphate for Ca2+-dependent inactivation of TRPC7 channel1
来源期刊 中国药理学报(英文版) 学科
关键词 canonical transient receptor potential 7 calcium inactivation,diacylglycerol inositol 1,4,5 trisphosphate
年,卷(期) 2008,(1) 所属期刊栏目
研究方向 页码范围 90-97
页数 8页 分类号
字数 语种 英文
DOI 10.1111/j.1745-7254.2008.00721.x
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研究主题发展历程
节点文献
canonical transient receptor potential 7
calcium
inactivation,diacylglycerol
inositol 1,4,5 trisphosphate
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期刊影响力
中国药理学报(英文版)
月刊
1671-4083
31-1347/R
大16开
上海市太原路294号
4-295
1980
eng
出版文献量(篇)
4416
总下载数(次)
2
总被引数(次)
42236
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